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Andrew J. Grosovsky
Professor of Cell Biology and Toxicologist
Email: andrew.grosovsky@ucr.edu
Molecular genetics of human cell response to environmental mutagens and carcinogens. Molecular mechanisms of genetic variability.
Research in my laboratory focuses on the mechanisms of mutagenesis and genomic instability in human cells. Genomic instability refers to an elevated rate of acquisition of new genetic alterations, and it has emerged as an important focus of contemporary carcinogenesis research. Our recent investigations of genomic instability suggest a model involving critical chromosomal aberrations which contain centromeric or pericentromeric heterochromatin at the rearrangement junction. These efforts are integrated with studies of fundamental mechanisms of mutagenesis, and the potential genotoxic effects of public exposure to environmental carcinogens. Recent studies have used molecular mapping and cytogenetic approaches to characterize chromosomal scale deletion and recombination events, as well as intragenic gene conversion and DNA sequence alterations. These techniques have permitted the identification of characteristic "mutational fingerprints" which may permit the retrospective identification of environmental carcinogen exposure responsible for induction of specific tumors in human populations.
Selected Publications:
- Giver, C.R. & A.J. Grosovsky. 1997. Single and Coincident Intragenic Mutations Attributable to Gene Conversion in a Human Cell Line. Genetics 146:1429-1439.
- Pongsaensook, P., L.E. Smith & A.J. Grosovsky. 1997. Isolation of an APRT Heterozygote from TK6 Human Lymphoblasts: Predominance of Multi-Locus Loss of Heterozygosity among Spontaneous APRT- Mutants. Mutation Res. 377:27-36.
- Grosovsky, A., K. Parks, C. Giver & S. Nelson. 1996. Clonal Analysis of Delayed Karyotypic Abnormalities and Gene Mutations in Radiation Induced Genetic Instability. Molec & Cell. Biol. 16:6252-6262.
- Nelson, S., K. Parks & A. Grosovsky. 1996. Ionizing radiation signature mutations in human cell mutants induced by low dose exposures. Mutagenesis 11:275-279.
- Giver, C.R., S.L. Nelson, Jr., M.Y. Cha, P. Pongsaensook & A.J. Grosovsky. 1995. Mutational spectrum of X-ray induced TK- human cell mutants. Carcinogenesis 16:267-275.
- Dobo, K.L., C.R. Giver, D.A. Eastmond, H.S. Rumbos & A.J. Grosovsky. 1995. Extensive loss of heterozygosity accounts for differential mutation rate on chromosome 17q in human lymphoblasts. Mutagenesis 10:53-58.
- Nelson, S.L., I.M. Jones, J.C. Fuscoe, K. Burkhart-Schultz & A.J. Grosovsky. 1995. Mapping the endpoints of large deletions affecting the hprt locus in human peripheral blood cells and cell lines. Radiation Research 141:2-10.
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